Abstract: Effect of Complex Amino Acid Imbalance Solutions on Hepatocarcinoma in Tumor Bearing H22MiceZhongfang YAN1,Wei LU2, Yumei QI3, Jing YANG3, Yukun JIN1, Shaocheng QIAN3Correspondence to:Wei LU, E-mail: luwei1966@126.com1Department of Nutrition, Haihe Hospital of Tianjin, Tianjin 300350, China2Department of Nutrition, Tianjin Municipal Hospital of Infectious Diseases, Tianjin 300192, China3Department of Nutrition, The Third Central Hospital of Tianjin, Tianjin 300170, ChinaThis work was supported by Natural Science Foundation of Tianjin Bureau of Public Health (No. 05KY15)Abstract Objectives: To study the influence of several amino acid imbalance solutions on hepatocarcinoma inhibition and nutri-tional status in tumor-bearing mice. Methods: Based on individual formulas of amino acid compositions in nutrient solutions, the hepa-toma22-bearing mice were divided into 9 groups: group A ( amino acid balance solution ), group B (a rginine-enriched amino acid im-balance solution), group C ( valine-depleted amino acid imbalance solution ), group D ( leucine-enriched amino acid imbalance solu-tion ), group E (tyrosine-depleted amino acid imbalance solution ), group F ( phenylalanine-depleted amino acid imbalance solution ),group G ( serine-depleted amino acid imbalance solution ), group H ( no amino acid ), and group I ( compound amino acid imbalancesolution ). Gastrointestinal nutrient solution was used for 12 days in the different groups of hepatoma22-bearing mice. Indices of hepato-carcinoma inhibition included tumor volume, tumor weight, tumor weight /carcass body weight, tumor cell necrosis rate, PCNA expres-sion and DNA ploidy. Results: The factors used to assess tumor inhibition were significantly lower in groups B, C, D, E, F, G, H and Ithan in group A ( P < 0.05 ), except for tumor cell necrosis and DNA ploidy ( P > 0.05 ). Cell cycle analysis showed that in a compari-son of group H and I with group A, the percentage of cells in G0/G1 phase was increased in group H and I, while the percentage of cellsin S phase and G2/M phase was decreased in the two groups ( P < 0.01 ). Conclusion: Balanced amino acid solution can stimulate tu-mor growth, while the complex amino acid imbalance solutions and some individual amino acid imbalance solutions have inhibitory ef-fect on hepatocarcinoma in nude mice.Keywords Amino acid imbalance solutions; Enteral nutrition; Mice; Hepatoma